Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of anti- Foxp3 Antibody (A00011-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse thymus tissue lysates,
Use rabbit anti- Foxp3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Foxp3 at approximately 47KD. The expected band size for Foxp3 is at 47KD.
Figure 1. Western blot analysis of anti- Foxp3 Antibody (A00011-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse thymus tissue lysates,
Use rabbit anti- Foxp3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Foxp3 at approximately 47KD. The expected band size for Foxp3 is at 47KD.