Figure 1. Western blot analysis of Caspase8 using anti-Caspase8 antibody (A00042). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates,Lane 3: HEPG2 whole cell lysates. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Caspase8 at approximately 55KD. The expected band size for Caspase8 is at 55KD.
all(8)
Figure 2. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of mouse spleen tissues Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(8) 
Figure 3. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of rat intestine tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(8) 
Figure 4. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of rat spleen tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(8) 
Figure 5. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of human intestinal cancer tissues. . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(8) 
Figure 6. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of human mammary cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(8) 
Figure 7. Flow Cytometry analysis of PC-3 cells using anti-CASP8 antibody (A00042).Overlay histogram showing PC-3 cells stained with A00042 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(8) 
Figure 8. Flow Cytometry analysis of Hela cells using anti-CASP8 antibody (A00042).Overlay histogram showing Hela cells stained with A00042 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(8) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of Caspase8 using anti-Caspase8 antibody (A00042). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates,Lane 3: HEPG2 whole cell lysates. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Caspase8 at approximately 55KD. The expected band size for Caspase8 is at 55KD.
Figure 2. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of mouse spleen tissues Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of rat intestine tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of rat spleen tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of human intestinal cancer tissues. . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of human mammary cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 7. Flow Cytometry analysis of PC-3 cells using anti-CASP8 antibody (A00042).Overlay histogram showing PC-3 cells stained with A00042 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 8. Flow Cytometry analysis of Hela cells using anti-CASP8 antibody (A00042).Overlay histogram showing Hela cells stained with A00042 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 1. Western blot analysis of Caspase8 using anti-Caspase8 antibody (A00042). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates,Lane 3: HEPG2 whole cell lysates. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Caspase8 at approximately 55KD. The expected band size for Caspase8 is at 55KD.
Figure 2. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of mouse spleen tissues Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of rat intestine tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of rat spleen tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of human intestinal cancer tissues. . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of Caspase8 using anti-Caspase8 antibody (A00042).Caspase8 was detected in paraffin-embedded section of human mammary cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 7. Flow Cytometry analysis of PC-3 cells using anti-CASP8 antibody (A00042).Overlay histogram showing PC-3 cells stained with A00042 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 8. Flow Cytometry analysis of Hela cells using anti-CASP8 antibody (A00042).Overlay histogram showing Hela cells stained with A00042 (Blue line).. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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