Western blot (WB): | 1:500-2000 |
ELISA(Cap): | 1:50-1:200 |
Figure 1. Western blot analysis of CCL2 using anti-CCL2 antibody (A00056-4). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for CCL2 at approximately 11KD. The expected band size for CCL2 is at 11KD.
Figure 1. Western blot analysis of CCL2 using anti-CCL2 antibody (A00056-4). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for CCL2 at approximately 11KD. The expected band size for CCL2 is at 11KD.