Western blot analysis of HMGB1 using anti-HMGB1 antibody (A00066-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMGB1 antigen affinity purified polyclonal antibody (A00066-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HMGB1 at approximately 25 kDa. The expected band size for HMGB1 is at 25 kDa.
Western blot analysis of HMGB1 using anti-HMGB1 antibody (A00066-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7- WT whole cell lysates,
Lane 2: mouse RAW264.7-Hmgb1 KO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMGB1 antigen affinity purified polyclonal antibody (A00066-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HMGB1 at approximately 25 kDa. The expected band size for HMGB1 is at 25 kDa.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).
HMGB1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).
HMGB1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of THP-1 cells using anti-HMGB1 antibody (A00066-1).
Overlay histogram showing THP-1 cells stained with A00066-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HMGB1 Antibody (A00066-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of HMGB1 using anti-HMGB1 antibody (A00066-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMGB1 antigen affinity purified polyclonal antibody (A00066-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HMGB1 at approximately 25 kDa. The expected band size for HMGB1 is at 25 kDa.
Western blot analysis of HMGB1 using anti-HMGB1 antibody (A00066-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7- WT whole cell lysates,
Lane 2: mouse RAW264.7-Hmgb1 KO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMGB1 antigen affinity purified polyclonal antibody (A00066-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HMGB1 at approximately 25 kDa. The expected band size for HMGB1 is at 25 kDa.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).
HMGB1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).
HMGB1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of THP-1 cells using anti-HMGB1 antibody (A00066-1).
Overlay histogram showing THP-1 cells stained with A00066-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HMGB1 Antibody (A00066-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of HMGB1 using anti-HMGB1 antibody (A00066-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMGB1 antigen affinity purified polyclonal antibody (A00066-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HMGB1 at approximately 25 kDa. The expected band size for HMGB1 is at 25 kDa.
Western blot analysis of HMGB1 using anti-HMGB1 antibody (A00066-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7- WT whole cell lysates,
Lane 2: mouse RAW264.7-Hmgb1 KO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMGB1 antigen affinity purified polyclonal antibody (A00066-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HMGB1 at approximately 25 kDa. The expected band size for HMGB1 is at 25 kDa.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of HMGB1 using anti-HMGB1 antibody (A00066-1) .
HMGB1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).
HMGB1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of HMGB1 using anti-HMGB1 antibody (A00066-1).
HMGB1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-HMGB1 Antibody (A00066-1) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of THP-1 cells using anti-HMGB1 antibody (A00066-1).
Overlay histogram showing THP-1 cells stained with A00066-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HMGB1 Antibody (A00066-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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