Anti-TRAF6 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM ELISA

A00185

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of TRAF6 using anti-TRAF6 antibody (A00185).
Lane 1: rat RH35 whole cell lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse RAW246.7 whole cell lysates.
anti-TRAF6 antigen affinity purified polyclonal antibody (Catalog # A00185)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for TRAF6 at approximately 60-65KD. The expected band size for TRAF6 is at 60KD.
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Figure 2. IHC analysis of TRAF6 using anti-TRAF6 antibody (A00185).
TRAF6 was detected in paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of TRAF6 using anti-TRAF6 antibody (A00185).
TRAF6 was detected in paraffin-embedded section of mouse intestine tissue. . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of HEPA 1-6 cells using anti-TRAF6 antibody (A00185).
Overlay histogram showing HEPA 1-6 cells stained with A00185 (Blue line).DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-TRAF6 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

RING finger protein 85; RNF85; TRAF6

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, FCM, ELISA

基因名称

Traf6

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse TRAF6 recombinant protein (Position: Q14-D512).

计算分子量

60 kDa

实际分子量

60 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

TNF receptor-associated factor 6, also called E3 ubiquitin-protein ligase TRAF6 or RNF85, is a TRAF human protein. The protein encoded by this gene is a member of the TNF receptor associated factor(TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This gene is mapped to 11p12. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase(IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein.

Uniprot ID

P70196

RRID

AB_3081003

文献引用格式

TRAF6 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00185)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

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