Anti-XBP1S-specific Antibody

50μl/1280 100μl/2180 150μl/2800

TREB5; X box binding protein 1; XBP 1; XBP1; XBP1s; XBP-1S; XBP1U; XBP2

Polyclonal

human, mouse

WB, IHC, ELISA

XBP1

Rabbit

IgG

E.coli-derived human XBP1S-specific recombinant protein (Position: A165-I374).

29 kDa

29 kDa

500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol.

Immunogen affinity purified.

500 ug/ml

Liquid

12 months from date of receipt，-20℃ as supplied.

XBP1, also known as X-box binding protein 1, is a protein which in humans is encoded by the XBP1 gene. It encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

P17861  

XBP1S-specific Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00234-2)

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

X-box binding protein 1

X-box-binding protein 1

Western blot (WB):	1:500-2000
Immunohistochemistry (IHC):	1:50-400
Enzyme linked immunosorbent assay (ELISA):	1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.