Anti-TNFR1/TNFRSF1A Antibody

筛选器： All WB FCM

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  Western blot analysis of TNFR1/TNFRSF1A using anti-TNFR1/TNFRSF1A antibody (A00294-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HepG2 whole cell lysates,
  Lane 2: HCCT whole cell lysates,
  Lane 3: HCCP whole cell lysates,
  Lane 4: HL-60 whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: C6 whole cell lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: Neuro-2a whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TNFR1/TNFRSF1A antigen affinity purified polyclonal antibody (A00294-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TNFR1/TNFRSF1A at approximately 55-60 kDa. The expected band size for TNFR1/TNFRSF1A is at 50 kDa.

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  Flow Cytometry analysis of U937 cells using anti-TNFR1/TNFRSF1A antibody (A00294-4).
  Overlay histogram showing U937 cells stained with A00294-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TNFR1/TNFRSF1A Antibody (A00294-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of TNFR1/TNFRSF1A using anti-TNFR1/TNFRSF1A antibody (A00294-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HepG2 whole cell lysates,
  Lane 2: HCCT whole cell lysates,
  Lane 3: HCCP whole cell lysates,
  Lane 4: HL-60 whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: C6 whole cell lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: Neuro-2a whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TNFR1/TNFRSF1A antigen affinity purified polyclonal antibody (A00294-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TNFR1/TNFRSF1A at approximately 55-60 kDa. The expected band size for TNFR1/TNFRSF1A is at 50 kDa.

• 

  Flow Cytometry analysis of U937 cells using anti-TNFR1/TNFRSF1A antibody (A00294-4).
  Overlay histogram showing U937 cells stained with A00294-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TNFR1/TNFRSF1A Antibody (A00294-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.