Figure 1. Western blot analysis of anti- CD4 antibody (A00344-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Mouse RAW264.7 whole cell lysates,Lane 2: Mouse ANA-1 whole cell lysates.Use rabbit anti- CD4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD4 at approximately 52KD. The expected band size for CD4 is at 52KD.
all(4)
Figure 2. IHC analysis using anti- CD4 antibody (A00344-2). detected in paraffin-embedded section of mouse thymus tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(4) 
Figure 3. Flow cytometry analysis of mouse PBMC cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(4) 
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Figure 1. Western blot analysis of anti- CD4 antibody (A00344-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Mouse RAW264.7 whole cell lysates,Lane 2: Mouse ANA-1 whole cell lysates.Use rabbit anti- CD4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD4 at approximately 52KD. The expected band size for CD4 is at 52KD.
Figure 2. IHC analysis using anti- CD4 antibody (A00344-2). detected in paraffin-embedded section of mouse thymus tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow cytometry analysis of mouse PBMC cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- CD4 antibody (A00344-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Mouse RAW264.7 whole cell lysates,Lane 2: Mouse ANA-1 whole cell lysates.Use rabbit anti- CD4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD4 at approximately 52KD. The expected band size for CD4 is at 52KD.
Figure 2. IHC analysis using anti- CD4 antibody (A00344-2). detected in paraffin-embedded section of mouse thymus tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow cytometry analysis of mouse PBMC cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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