Figure 1. Western blot analysis of anti-ATG7 antibody (A00346). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human CACO-2 whole cell lysates,Lane 2: human U2OS whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human HEK293 whole cell lysates,Lane 5: human HELA whole cell lysates,Lane 6: human K562 whole cell lysates,Lane 7: Rat thymus tissue lysates,Lane 8: Rat spleen tissue lysates,Lane 9:Mouse SP2/0 whole cell lysates,Use rabbit anti- ATG7 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATG7 at approximately 78KD. The expected band size for ATG7 is at 78KD.
all(6)
Figure 2. Flow cytometry analysis of C6 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(6) 
Figure 3. Flow cytometry analysis of HELA cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(6) 
Figure 4. Flow cytometry analysis of mouse RAW264.7 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(6) 
Figure 5. IHC analysis using anti- ATG7 antibody (A00346). detected in paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 6. IHC analysis using anti- ATG7 antibody (A00346). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(6) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti-ATG7 antibody (A00346). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human CACO-2 whole cell lysates,Lane 2: human U2OS whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human HEK293 whole cell lysates,Lane 5: human HELA whole cell lysates,Lane 6: human K562 whole cell lysates,Lane 7: Rat thymus tissue lysates,Lane 8: Rat spleen tissue lysates,Lane 9:Mouse SP2/0 whole cell lysates,Use rabbit anti- ATG7 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATG7 at approximately 78KD. The expected band size for ATG7 is at 78KD.
Figure 2. Flow cytometry analysis of C6 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 3. Flow cytometry analysis of HELA cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 4. Flow cytometry analysis of mouse RAW264.7 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 5. IHC analysis using anti- ATG7 antibody (A00346). detected in paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis using anti- ATG7 antibody (A00346). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. Western blot analysis of anti-ATG7 antibody (A00346). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human CACO-2 whole cell lysates,Lane 2: human U2OS whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human HEK293 whole cell lysates,Lane 5: human HELA whole cell lysates,Lane 6: human K562 whole cell lysates,Lane 7: Rat thymus tissue lysates,Lane 8: Rat spleen tissue lysates,Lane 9:Mouse SP2/0 whole cell lysates,Use rabbit anti- ATG7 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATG7 at approximately 78KD. The expected band size for ATG7 is at 78KD.
Figure 2. Flow cytometry analysis of C6 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 3. Flow cytometry analysis of HELA cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 4. Flow cytometry analysis of mouse RAW264.7 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 5. IHC analysis using anti- ATG7 antibody (A00346). detected in paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis using anti- ATG7 antibody (A00346). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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