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Anti-CD11c/Integrin Alpha X/ITGAX Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ELISA

A00357-3

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of CD11c/Integrin Alpha X/ITGAX using anti-CD11c/Integrin Alpha X/ITGAX antibody (A00357-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: mouse lung tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD11c/Integrin Alpha X/ITGAX antigen affinity purified polyclonal antibody (A00357-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD11c/Integrin Alpha X/ITGAX at approximately 128,150 kDa. The expected band size for CD11c/Integrin Alpha X/ITGAX is at 129 kDa.

    all(5)
  • IHC analysis of CD11c/Integrin Alpha X/ITGAX using anti-CD11c/Integrin Alpha X/ITGAX antibody (A00357-3).
    CD11c/Integrin Alpha X/ITGAX was detected in a paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD11c/Integrin Alpha X/ITGAX Antibody (A00357-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IHC analysis of CD11c/Integrin Alpha X/ITGAX using anti-CD11c/Integrin Alpha X/ITGAX antibody (A00357-3).
    CD11c/Integrin Alpha X/ITGAX was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD11c/Integrin Alpha X/ITGAX Antibody (A00357-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IHC analysis of CD11c/Integrin Alpha X/ITGAX using anti-CD11c/Integrin Alpha X/ITGAX antibody (A00357-3).
    CD11c/Integrin Alpha X/ITGAX was detected in a paraffin-embedded section of rat Lymph node tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD11c/Integrin Alpha X/ITGAX Antibody (A00357-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(5)

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产品名称
Anti-CD11c/Integrin Alpha X/ITGAX Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CD11C; Integrin alpha X; ITGAX; Leu M5; SLEB6
产品类型
Polyclonal
检验物种
mouse, rat
应用范围
WB, IHC, ELISA
基因名称
Itgax
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived mouse CD11c/Itgax recombinant protein (Position: P140-T342).
计算分子量
129 kDa
实际分子量
128,150 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
CD11c, also known as Integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a genethat encodes for CD11c. This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene.
Uniprot ID
Q9QXH4  
RRID
文献引用格式
CD11c/Integrin Alpha X/ITGAX Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00357-3)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
基因名全称
integrin alpha X
蛋白名全称
Integrin alpha-X
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC): 1:50-400
Enzyme linked immunosorbent assay (ELISA):1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-CD11c/Integrin Alpha X/ITGAX Antibody

    筛选器: All WB IHC ELISA

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