Anti-iNOS/NOS2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

A00368

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of iNOS using anti-iNOS antibody (A00368). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates.-iNOS antigen affinity purified polyclonal antibody (Catalog # A00368) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for iNOS at approximately 130KD. The expected band size for iNOS is at 130KD.
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产品名称

Anti-iNOS/NOS2 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

HEP NOS; Hepatocyte NOS; Inducible NO synthase; Inducible NOS; INOS; NOS; NOS type II; NOS2; NOS2A

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

NOS2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human iNOS, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

计算分子量

131 kDa

实际分子量

133 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Nitric oxide synthase, inducible is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter; it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. Three different NOS isoforms have been identified which fall into two distinct types, constitutive and inducible. The inducible NOS (iNOS) isoform is expressed in a variety of cell types and tissues in response to inflammatory agents and cytokines. The human iNOS (NOS2) gene is approximately 37 kb in length and consists of 26 exons and 25 introns. NOS2-derived NO is a prerequisite for cytokine signaling and function in innate immunity.

Uniprot ID

P35228

RRID

AB_3081084

文献引用格式

iNOS/NOS2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00368)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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