ELISA: | 1:100-1000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
IHC analysis of MIP-1 Alpha/CCL3 using anti-MIP-1 Alpha/CCL3 antibody (A00405-3).
MIP-1 Alpha/CCL3 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MIP-1 Alpha/CCL3 Antibody (A00405-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MIP-1 Alpha/CCL3 using anti-MIP-1 Alpha/CCL3 antibody (A00405-3).
MIP-1 Alpha/CCL3 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MIP-1 Alpha/CCL3 Antibody (A00405-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MIP-1 Alpha/CCL3 using anti-MIP-1 Alpha/CCL3 antibody (A00405-3).
MIP-1 Alpha/CCL3 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MIP-1 Alpha/CCL3 Antibody (A00405-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MIP-1 Alpha/CCL3 using anti-MIP-1 Alpha/CCL3 antibody (A00405-3).
MIP-1 Alpha/CCL3 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MIP-1 Alpha/CCL3 Antibody (A00405-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.