Anti-BCRP/ABCG2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM ELISA

A00457-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of BCRP/ABCG2 using anti-BCRP/ABCG2 antibody (A00457-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human MDA-MB-453 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: Rat liver tissue lysates,
Lane 7: Rat kidney tissue lysates,
Lane 8: Mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BCRP/ABCG2 antigen affinity purified polyclonal antibody (A00457-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BCRP/ABCG2 at approximately 65-80 kDa. The expected band size for BCRP/ABCG2 is at 72 kDa.
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IHC analysis of BCRP/ABCG2 using anti-BCRP/ABCG2 antibody (A00457-2).
BCRP/ABCG2 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BCRP/ABCG2 Antibody (A00457-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of BCRP/ABCG2 using anti-BCRP/ABCG2 antibody (A00457-2).
BCRP/ABCG2 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BCRP/ABCG2 Antibody (A00457-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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Flow Cytometry analysis of SiHa cells using anti-BCRP/ABCG2 antibody (A00457-2).
Overlay histogram showing SiHa cells stained with A00457-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCRP/ABCG2 Antibody (A00457-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-BCRP/ABCG2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ABC15; ABCG2; ABCP; BCRP; ABCG2; BMDP; CD338; CDw338; EST157481; MRX; MXR; MXR1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM, ELISA

基因名称

ABCG2

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human BCRP/ABCG2 recombinant protein (Position: M1-R378). Human ABCG2 shares 80.5% and 79.34% amino acid (aa) sequence identity with mouse and rat ABCG2, respectively.

计算分子量

72 kDa

实际分子量

65-80 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ABCG2(Atp-binding cassette, subfamily g, member 2) also known as ABCP, BCRP or MRX, is a protein that in humans is encoded by the ABCG2 gene. It is mapped on 4q22.1. The ABCG2 gene encodes a membrane transporter belonging to the ATP-binding cassette (ABC) superfamily of membrane transporters, which are involved in the trafficking of biologic molecules across cell membranes. The ABCG2 protein is also a high capacity transporter for uric acid excretion in the kidney, liver, and gut. In vitro assays of isolated membrane preparations revealed a high-capacity, vanadate-sensitive ATPase activity associated with ABCG2 expression that was stimulated by compounds known to be transported by this protein. ABCG2 is likely functioning as a homodimer or homooligomer in this expression system since it is unlikely that putative Sf9 transport partners would be overexpressed at similarly high levels. Abcg2 transports pheophorbide-a, which occurs in various plant-derived foods and food supplements and is highly efficient in limiting its uptake from ingested food. ABCG2 is a major factor in the concentrative transfer of drugs, carcinogens, and dietary toxins to the milk of mice, cows, and humans.

Uniprot ID

Q9UNQ0

RRID

AB_3081129

文献引用格式

BCRP/ABCG2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00457-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ATP-binding cassette, sub-family G (WHITE), member 2

蛋白名全称

ATP-binding cassette sub-family G member 2

实验方案>

实验步骤

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