Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of anti- NOD1 antibody (A00495-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: monkey heart tissue lysates,Lane 2: monkey skeletal muscle tissue lysates.Use rabbit anti- NOD1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for NOD1 at approximately 107KD. The expected band size for NOD1 is at 107KD.
Figure 1. Western blot analysis of anti- NOD1 antibody (A00495-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: monkey heart tissue lysates,Lane 2: monkey skeletal muscle tissue lysates.Use rabbit anti- NOD1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for NOD1 at approximately 107KD. The expected band size for NOD1 is at 107KD.