Anti-Apolipoprotein AI/APOA1 DyLight 488 Conjugated Antibody

Rabbit polyclonal antibody

说明书

筛选器： All FCM

A00717-Dyl488

100μl ￥2380
货期： 5-7天

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Figure 1. Flow Cytometry analysis of HEPG2 cells using anti-Human Apolipoprotein A I antibody (A00717-Dyl488).Overlay histogram showing HEPG2 cells stained with A00717-Dyl488 (Blue line). Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品名称

Anti-Apolipoprotein AI/APOA1 DyLight 488 Conjugated Antibody

规格/价格

100μl/2380

指标别称

Apo AI; ApoA I; APOA1; apolipoprotein A I; Apolipoprotein A1

产品类型

Polyclonal

检验物种

human

应用范围

FCM

基因名称

APOA1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human Apolipoprotein A I recombinant protein (Position: D25-Q267). Human Apolipoprotein A I shares 64% and 61.7% amino acid (aa) sequence identity with mouse and rat Apolipoprotein A I, respectively.

偶联物

DyLight 488

激发/发射光谱

Amax=488nm; Emax=515-545nm

成分

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

纯化方式

Immunogen affinity purified.

浓度

500ug/ml

产品形态

Liquid

保存条件

At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

背景资料

Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL. The gene is mapped to 11q23. And it is a single polypeptide chain with 243 amino acid residues of known primary amino acid sequence. The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.

Uniprot ID

P02647

文献引用格式

Apolipoprotein AI/APOA1 DyLight 488 Conjugated Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00717-Dyl488)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

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实验步骤

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