Anti-SMAD7 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A00784-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SMAD7 using anti-SMAD7 antibody (A00784-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMAD7 antigen affinity purified polyclonal antibody (A00784-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SMAD7 at approximately 50 kDa. The expected band size for SMAD7 is at 46 kDa.
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产品名称

Anti-SMAD7 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CRCS3; hSMAD7; MAD homolog 7; MAD homolog 8; MADH7; MADH8; Mothers against DPP homolog 7; Mothers against DPP homolog 8; SMAD 7; SMAD family member 7; SMAD7

产品类型

Polyclonal

检验物种

human

应用范围

WB, ELISA

基因名称

SMAD7

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human MADH7/SMAD7 recombinant protein (Position: R353-Q388).

计算分子量

46 kDa

实际分子量

50 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Mothers against decapentaplegic homolog 7 or SMAD7 is a protein that in humans is encoded by the SMAD7 gene. The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

O15105

RRID

AB_3081240

文献引用格式

SMAD7 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00784-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

SMAD family member 7

蛋白名全称

Mothers against decapentaplegic homolog 7

实验方案>

实验步骤

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