Figure 1. Western blot analysis of anti-FGA antibody (A00816-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human T-47D whole cell lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human SW620 whole cell lysates.Use rabbit anti- FGA 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for FGA at approximately 95KD. The expected band size for FGA is at 95KD.
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Figure 2. IHC analysis of anti-FGA antibody (A00816-3).detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Flow cytometry analysis of HepG2 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
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Figure 4. Flow cytometry analysis of SiHa cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
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| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti-FGA antibody (A00816-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human T-47D whole cell lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human SW620 whole cell lysates.Use rabbit anti- FGA 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for FGA at approximately 95KD. The expected band size for FGA is at 95KD.
Figure 2. IHC analysis of anti-FGA antibody (A00816-3).detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow cytometry analysis of HepG2 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
Figure 4. Flow cytometry analysis of SiHa cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti-FGA antibody (A00816-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human T-47D whole cell lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human SW620 whole cell lysates.Use rabbit anti- FGA 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for FGA at approximately 95KD. The expected band size for FGA is at 95KD.
Figure 2. IHC analysis of anti-FGA antibody (A00816-3).detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow cytometry analysis of HepG2 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
Figure 4. Flow cytometry analysis of SiHa cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
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