Anti-NNT Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF FCM ELISA

A00887-3

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-NNT antibody (A00887-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat liver tissue lysates,Lane 2: Rat kidney tissue lysates,Lane 3: Rat heart tissue lysates,Lane 4: Mouse liver tissue lysates,Lane 5: Mouse kidney tissue lysates,Lane 6: Mouse heart tissue lysates,Lane 7: human HELA whole cell lysates,Lane 8: Monkey liver tissue lysates,Lane 9: Monkey kidney tissue lysates,Use rabbit anti- NNT 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for NNT at approximately 114KD. The expected band size for NNT is at 114KD.
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Figure 2. ICC analysis using anti-NNT antibody (A00887-3) was detected in immersion fixed U2OS cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue).
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Figure 3. Flow cytometry analysis of THP-1 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-NNT Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

NNT

产品类型

Polyclonal

检验物种

human, monkey, mouse, rat

应用范围

WB, ICC/IF, FCM, ELISA

基因名称

NNT

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human NNT recombinant protein (Position: C44-A350).

计算分子量

114 kDa

实际分子量

114 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification.

Uniprot ID

Q13423

RRID

AB_3081275

文献引用格式

NNT Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00887-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

nicotinamide nucleotide transhydrogenase

蛋白名全称

NAD(P) transhydrogenase, mitochondrial

实验方案>

实验步骤

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