Anti-IGFBP1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA(Cap)

A00922-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of IGFBP1 using anti-IGFBP1 antibody (A00922-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IGFBP1 antigen affinity purified polyclonal antibody (A00922-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IGFBP1 at approximately 30 kDa. The expected band size for IGFBP1 is at 30 kDa.
all(6)
IHC analysis of IGFBP1 using anti-IGFBP1 antibody (A00922-1).
IGFBP1 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-IGFBP1 Antibody (A00922-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of IGFBP1 using anti-IGFBP1 antibody (A00922-1).
IGFBP1 was detected in a paraffin-embedded section of mouse pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-IGFBP1 Antibody (A00922-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of IGFBP1 using anti-IGFBP1 antibody (A00922-1).
IGFBP1 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-IGFBP1 Antibody (A00922-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of IGFBP1 using anti-IGFBP1 antibody (A00922-1).
IGFBP1 was detected in a paraffin-embedded section of rat pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-IGFBP1 Antibody (A00922-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
all(6)

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产品简介>

产品名称

Anti-IGFBP1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AFBP; hIGFBP 1; IBP 1; IBP1; IGF binding protein 1; IGF BP25; IGFBP 1; IGFBP1; Placental protein 12; PP12

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, ELISA(Cap)

基因名称

Igfbp1

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived mouse IGFBP1 recombinant protein (Position: A26-N272). Mouse IGFBP1 shares 68.4% and 95.5% amino acid (aa) sequences identity with human and rat IGFBP1, respectively.

计算分子量

30 kDa

实际分子量

30 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

IGFBP1, Insulin-like growth factor-binding protein 1, also known as placental protein 12 (PP12), is a protein that in humans is encoded by the IGFBP1 gene. The IGFBP1 gene has 4 exons and spans 5.9 kb. And the IGFBP1 gene is localized to 7p13-p12 by in situ hybridization. This gene is a member of the Insulin-like growth factor-binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a type-I thyroglobulin domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Uniprot ID

P47876

RRID

AB_3081289

文献引用格式

IGFBP1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00922-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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