Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Flow Cytometry (Fixed): | 1:50-200 |
Figure 1. Western blot analysis of anti- EIF4EBP1 Antibody (A00968-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HEL whole cell lysates,
Lane 2: PC-3 whole cell lysates,
Lane 3: HEK293 whole cell lysates,
Lane 4: rat stomach tissue lysates,
Lane 5: rat pancreas tissue lysates,
Lane 6: mouse pancreas tissue lysates.
Use rabbit anti- EIF4EBP1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for EIF4EBP1 at approximately 17KD. The expected band size for EIF4EBP1 is at 13KD.
Figure 2. IHC analysis using anti- EIF4EBP1 Antibody (A00968-1). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow cytometry analysis of A431 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).