Anti-AMPK Alpha 1/PRKAA1 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB ICC/IF FCM ELISA

A00994-3

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of AMPK alpha 1 using anti-AMPK alpha 1 antibody (A00994-3). Lane 1: human PANC-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates. anti-AMPK alpha 1 antigen affinity purified polyclonal antibody (Catalog # A00994-3)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for AMPK alpha 1 at approximately 64KD. The expected band size for AMPK alpha 1 is at 64KD.
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Figure 2. ICC analysis of anti- PRKAA1 antibody (A00994-3).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue).
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Figure 3. Flow cytometry analysis of SiHa cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
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产品简介>

产品名称

Anti-AMPK Alpha 1/PRKAA1 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

AMPK; AMPK alpha; AMPK alpha 1; AMPK subunit alpha 1; AMPK1; AMPKa1; PRKAA1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, FCM, ELISA

基因名称

PRKAA1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human AMPK alpha 1 recombinant protein (Position: F377-R446).

计算分子量

64 kDa

实际分子量

64 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Uniprot ID

Q13131

文献引用格式

AMPK Alpha 1/PRKAA1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00994-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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