Figure 1. Western blot analysis of anti-LBR antibody (A01238-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human HEK293 whole cell lysates,
Lane 6: human U2OS whole cell lysates,
Lane 7: human U-937 whole cell lysates,
Lane 8: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LBR antigen affinity purified polyclonal antibody (A01238-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LBR at approximately 65-71 kDa. The expected band size for LBR is at 71 kDa.
Figure 2. Western blot analysis of anti-LBR antibody (A01238-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat testicular tissue lysates,
Lane 4: mouse thymus tissue lysates,
Lane 5: mouse stomach tissue lysates,
Lane 6: mouse testicular tissue lysates,
Lane 7: mouse SP20 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LBR antigen affinity purified polyclonal antibody (A01238-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LBR at approximately 65-71 kDa. The expected band size for LBR is at 71 kDa.
Figure 3. IHC analysis of LBR using anti-LBR antibody (A01238-2).
LBR was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IF analysis of LBR using anti-LBR antibody (A01238-2).
LBR was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 5. Flow Cytometry analysis of U2OS cells using anti-LBR antibody (A01238-2).
Overlay histogram showing U2OS cells stained with A01238-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LBR Antibody (A01238-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti-LBR antibody (A01238-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human HEK293 whole cell lysates,
Lane 6: human U2OS whole cell lysates,
Lane 7: human U-937 whole cell lysates,
Lane 8: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LBR antigen affinity purified polyclonal antibody (A01238-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LBR at approximately 65-71 kDa. The expected band size for LBR is at 71 kDa.
Figure 2. Western blot analysis of anti-LBR antibody (A01238-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat testicular tissue lysates,
Lane 4: mouse thymus tissue lysates,
Lane 5: mouse stomach tissue lysates,
Lane 6: mouse testicular tissue lysates,
Lane 7: mouse SP20 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LBR antigen affinity purified polyclonal antibody (A01238-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LBR at approximately 65-71 kDa. The expected band size for LBR is at 71 kDa.
Figure 3. IHC analysis of LBR using anti-LBR antibody (A01238-2).
LBR was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IF analysis of LBR using anti-LBR antibody (A01238-2).
LBR was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 5. Flow Cytometry analysis of U2OS cells using anti-LBR antibody (A01238-2).
Overlay histogram showing U2OS cells stained with A01238-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LBR Antibody (A01238-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 1. Western blot analysis of anti-LBR antibody (A01238-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human HEK293 whole cell lysates,
Lane 6: human U2OS whole cell lysates,
Lane 7: human U-937 whole cell lysates,
Lane 8: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LBR antigen affinity purified polyclonal antibody (A01238-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LBR at approximately 65-71 kDa. The expected band size for LBR is at 71 kDa.
Figure 2. Western blot analysis of anti-LBR antibody (A01238-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat testicular tissue lysates,
Lane 4: mouse thymus tissue lysates,
Lane 5: mouse stomach tissue lysates,
Lane 6: mouse testicular tissue lysates,
Lane 7: mouse SP20 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LBR antigen affinity purified polyclonal antibody (A01238-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LBR at approximately 65-71 kDa. The expected band size for LBR is at 71 kDa.
Figure 3. IHC analysis of LBR using anti-LBR antibody (A01238-2).
LBR was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IF analysis of LBR using anti-LBR antibody (A01238-2).
LBR was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 5. Flow Cytometry analysis of U2OS cells using anti-LBR antibody (A01238-2).
Overlay histogram showing U2OS cells stained with A01238-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LBR Antibody (A01238-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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