欢迎访问博士德生物官网! 服务热线:027-67845390 |
您当前的位置: 首页 > 产品中心 > 一抗 > 多克隆抗体

Anti-VEGFR3/FLT4 Antibody

Rabbit polyclonal antibody

说明书 一键复制产品信息

筛选器: All WB IHC ICC/IF FCM

A01276-2

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 现货
  • Figure 1. Western blot analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-2). Lane 1: human Hela whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human HepG2 whole cell lysates. anti-VEGF Receptor 3 antigen affinity purified polyclonal antibody (Catalog # A01276-2)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for VEGF Receptor 3 at approximately 153KD. The expected band size for VEGF Receptor 3 is at 153KD.

    all(10)
  • Figure 2. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-2).VEGF Receptor 3 was detected in paraffin-embedded section of human colon cancer tissue. anti-VEGF Receptor 3 Antibody (A01276-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(10)
  • Figure 3. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-2).VEGF Receptor 3 was detected in paraffin-embedded section of human lung cancer tissue. anti-VEGF Receptor 3 Antibody (A01276-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(10)
  • Figure 4. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-2).VEGF Receptor 3 was detected in paraffin-embedded section of human placenta tissue. anti-VEGF Receptor 3 Antibody (A01276-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(10)
  • Figure 5. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-2).VEGF Receptor 3 was detected in paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(10)
  • Figure 6. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-2).VEGF Receptor 3 was detected in paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(10)
  • Figure 7. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-2).VEGF Receptor 3 was detected in paraffin-embedded section of rat liver tissue. . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(10)
  • Figure 8. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-2).VEGF Receptor 3 was detected in paraffin-embedded section of human mammary cancer tissue. anti-VEGF Receptor 3 Antibody (A01276-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(10)
  • Figure 9. IF analysis of VEGF Receptor 3 using anti- VEGF Receptor 3 antibody (A01276-2).
    VEGF Receptor 3 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    all(10)
  • Figure 10. Flow Cytometry analysis of U2OS cells using anti- VEGF Receptor 3 antibody (A01276-2). Overlay histogram showing U2OS cells stained with A01276-2 (Blue line). DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

    all(10)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-VEGFR3/FLT4 Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
FLT 4; FLT4; FLT41; Fms like tyrosine kinase 4; fms related tyrosine kinase 4; LMPH1A; PCL; VEGFR 3; VEGFR3
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, FCM
基因名称
FLT4
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of human VEGF Receptor 3, which shares 82.8% and 79.3% amino acid (aa) sequence identity with mouse and rat VEGF Receptor 3, respectively.
计算分子量
153 kDa
实际分子量
153 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.
Uniprot ID
P35916  
文献引用格式
VEGFR3/FLT4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01276-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
Flow Cytometry (Fixed):1:50-200
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

实验方案>

实验步骤

引用文献(0)>

    相关产品>

    成功添加到购物车

    查看购物车 继续购物

    Anti-VEGFR3/FLT4 Antibody

    筛选器: All WB IHC ICC/IF FCM

    亮暗模式切换
    X
    亮暗模式切换
    X

    在线
    客服

    在线客服
    服务时间:08:30 - 17:30

    点击下列客服在线咨询:

    服务热线

    服务
    热线

    客服服务热线

    027-67845390
    客服服务热线

    扫码

    扫码
    咨询

    微信二维码 微信扫一扫立即咨询
    返回顶部 顶部