Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |
Figure 1. Western blot analysis of NCF1 using anti-NCF1 antibody (A01586-1).
Lane 1: human Hela whole cell lysates,
Lane 2: human blood lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse NIH3T3 whole cell lysates.
anti-NCF1 antigen affinity purified polyclonal antibody (Catalog # A01586-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for NCF1 at approximately 47KDThe expected band size for NCF1 is at 44KD.
Figure 1. Western blot analysis of NCF1 using anti-NCF1 antibody (A01586-1).
Lane 1: human Hela whole cell lysates,
Lane 2: human blood lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse NIH3T3 whole cell lysates.
anti-NCF1 antigen affinity purified polyclonal antibody (Catalog # A01586-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for NCF1 at approximately 47KDThe expected band size for NCF1 is at 44KD.