Figure 1. Western blot analysis of anti- YWHAE antibody (A01687-4). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human hepg2 whole cell lysates,Lane 4: human SH-SY5Y whole cell lysates,Lane 5: human HEK293 whole cell lysates,Lane 6: human SW620 whole cell lysates,Lane 7: human A549 whole cell lysates,Lane 8: human Raji whole cell lysates,Lane 9: Rat brain tissue lysates,Lane 10: Mouse brain tissue lysates,Lane 11: Mouse NIH/3T3 whole cell lysates,Lane 12: Mouse RAW264.7 whole cell lysates,Use rabbit anti- YWHAE 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAE at approximately 29KD. The expected band size for YWHAE is at 29KD.
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Figure 2. IHC analysis using anti- YWHAE antibody (A01687-4). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(5) 
Figure 4. ICC analysis using anti- YWHAE antibody (A01687-4). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
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| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- YWHAE antibody (A01687-4). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human hepg2 whole cell lysates,Lane 4: human SH-SY5Y whole cell lysates,Lane 5: human HEK293 whole cell lysates,Lane 6: human SW620 whole cell lysates,Lane 7: human A549 whole cell lysates,Lane 8: human Raji whole cell lysates,Lane 9: Rat brain tissue lysates,Lane 10: Mouse brain tissue lysates,Lane 11: Mouse NIH/3T3 whole cell lysates,Lane 12: Mouse RAW264.7 whole cell lysates,Use rabbit anti- YWHAE 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAE at approximately 29KD. The expected band size for YWHAE is at 29KD.
Figure 2. IHC analysis using anti- YWHAE antibody (A01687-4). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 4. ICC analysis using anti- YWHAE antibody (A01687-4). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
Figure 1. Western blot analysis of anti- YWHAE antibody (A01687-4). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human hepg2 whole cell lysates,Lane 4: human SH-SY5Y whole cell lysates,Lane 5: human HEK293 whole cell lysates,Lane 6: human SW620 whole cell lysates,Lane 7: human A549 whole cell lysates,Lane 8: human Raji whole cell lysates,Lane 9: Rat brain tissue lysates,Lane 10: Mouse brain tissue lysates,Lane 11: Mouse NIH/3T3 whole cell lysates,Lane 12: Mouse RAW264.7 whole cell lysates,Use rabbit anti- YWHAE 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAE at approximately 29KD. The expected band size for YWHAE is at 29KD.
Figure 2. IHC analysis using anti- YWHAE antibody (A01687-4). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 4. ICC analysis using anti- YWHAE antibody (A01687-4). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
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