Figure 1. Western blot analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD90/THY1 antigen affinity purified polyclonal antibody (A01818) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD90/THY1 at approximately 22 kDa. The expected band size for CD90/THY1 is at 18 kDa.
Figure 2. IHC analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818) .
CD90/THY1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (A01818) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818) .
CD90/THY1 was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (A01818) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818) .
CD90/THY1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (A01818) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818).
CD90/THY1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (A01818) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD90/THY1 antigen affinity purified polyclonal antibody (A01818) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD90/THY1 at approximately 22 kDa. The expected band size for CD90/THY1 is at 18 kDa.
Figure 2. IHC analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818) .
CD90/THY1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (A01818) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818) .
CD90/THY1 was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (A01818) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818) .
CD90/THY1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (A01818) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818).
CD90/THY1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (A01818) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 1. Western blot analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD90/THY1 antigen affinity purified polyclonal antibody (A01818) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD90/THY1 at approximately 22 kDa. The expected band size for CD90/THY1 is at 18 kDa.
Figure 2. IHC analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818) .
CD90/THY1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (A01818) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818) .
CD90/THY1 was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (A01818) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818) .
CD90/THY1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (A01818) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis of CD90/THY1 using anti-CD90/THY1 antibody (A01818).
CD90/THY1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CD90/THY1 Antibody (A01818) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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