Figure 1. Western blot analysis of anti- FIS1 antibody (A01932-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat brain tissue lysates,Lane 2: Rat spleen tissue lysates,Lane 3: Rat kidney tissue lysates,Lane 4: Mouse brain tissue lysates,Lane 5: Mouse spleen tissue lysates,Lane 6: Mouse kidney tissue lysates,Lane 7: Mouse NIH/3T3 whole cell lysates,Lane 8: Mouse RAW264.7 whole cell lysates,Lane 9: Human HEK293 whole cell lysates,Lane 10: Human A549 whole cell lysates,Lane 11: Human HELA whole cell lysates,Lane 12: Human Raji whole cell lysates,Lane 13: Human CACO-2 whole cell lysates,Use rabbit anti- FIS1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for FIS1 at approximately 17KD. The expected band size for FIS1 is at 17KD.
all(7)
Figure 2. IHC analysis using anti-FIS1 antibody (A01932-2). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(7) 
Figure 3. IHC analysis using anti-FIS1 antibody (A01932-2). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(7) 
Figure 4. IHC analysis using anti-FIS1 antibody (A01932-2). detected in paraffin-embedded section of human Ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(7) 
Figure 5. ICC analysis using anti-FIS1 antibody (A01932-2) was detected in immersion fixed HELA cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue).
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Figure 6. Flow cytometry analysis of A549 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- FIS1 antibody (A01932-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat brain tissue lysates,Lane 2: Rat spleen tissue lysates,Lane 3: Rat kidney tissue lysates,Lane 4: Mouse brain tissue lysates,Lane 5: Mouse spleen tissue lysates,Lane 6: Mouse kidney tissue lysates,Lane 7: Mouse NIH/3T3 whole cell lysates,Lane 8: Mouse RAW264.7 whole cell lysates,Lane 9: Human HEK293 whole cell lysates,Lane 10: Human A549 whole cell lysates,Lane 11: Human HELA whole cell lysates,Lane 12: Human Raji whole cell lysates,Lane 13: Human CACO-2 whole cell lysates,Use rabbit anti- FIS1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for FIS1 at approximately 17KD. The expected band size for FIS1 is at 17KD.
Figure 2. IHC analysis using anti-FIS1 antibody (A01932-2). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis using anti-FIS1 antibody (A01932-2). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis using anti-FIS1 antibody (A01932-2). detected in paraffin-embedded section of human Ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. ICC analysis using anti-FIS1 antibody (A01932-2) was detected in immersion fixed HELA cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue).
Figure 6. Flow cytometry analysis of A549 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- FIS1 antibody (A01932-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat brain tissue lysates,Lane 2: Rat spleen tissue lysates,Lane 3: Rat kidney tissue lysates,Lane 4: Mouse brain tissue lysates,Lane 5: Mouse spleen tissue lysates,Lane 6: Mouse kidney tissue lysates,Lane 7: Mouse NIH/3T3 whole cell lysates,Lane 8: Mouse RAW264.7 whole cell lysates,Lane 9: Human HEK293 whole cell lysates,Lane 10: Human A549 whole cell lysates,Lane 11: Human HELA whole cell lysates,Lane 12: Human Raji whole cell lysates,Lane 13: Human CACO-2 whole cell lysates,Use rabbit anti- FIS1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for FIS1 at approximately 17KD. The expected band size for FIS1 is at 17KD.
Figure 2. IHC analysis using anti-FIS1 antibody (A01932-2). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis using anti-FIS1 antibody (A01932-2). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis using anti-FIS1 antibody (A01932-2). detected in paraffin-embedded section of human Ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. ICC analysis using anti-FIS1 antibody (A01932-2) was detected in immersion fixed HELA cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue).
Figure 6. Flow cytometry analysis of A549 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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