Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |
Figure 1. Western blot analysis of anti-UBD antibody (A01970-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human K562 whole cell lysates.Use rabbit anti-UBD 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for UBD at approximately 18KD. The expected band size for UBD is at 18KD.
Figure 1. Western blot analysis of anti-UBD antibody (A01970-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human K562 whole cell lysates.Use rabbit anti-UBD 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for UBD at approximately 18KD. The expected band size for UBD is at 18KD.