Anti-tPA/PLAT Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM

A02965-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of tPA/PLAT using anti-tPA/PLAT antibody (A02965-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-tPA/PLAT antigen affinity purified polyclonal antibody (A02965-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for tPA/PLAT at approximately 63-69 kDa. The expected band size for tPA/PLAT is at 63 kDa.
all(3)
IHC analysis of tPA/PLAT using anti-tPA/PLAT antibody (A02965-1).
tPA/PLAT was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-tPA/PLAT Antibody (A02965-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
Flow Cytometry analysis of U251 cells using anti-PLAT antibody (A02965-1).Overlay histogram showing U251 cells stained with A02965-1 (Blue line).anti-PLAT Antibody (A02965-1, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(3)

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产品简介>

产品名称

Anti-tPA/PLAT Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alteplase; plasminogen activator; tissue; PLAT; Reteplase; T PA; t plasminogen activator; Tissue Plasminogen Activator; tPA

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM

基因名称

PLAT

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human PLAT, which shares 87.5% and 79.2% amino acid (aa) sequence identity with mouse and rat PLAT, respectively.

计算分子量

63 kDa

实际分子量

63-69 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PLAT is also known as tPA. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Uniprot ID

P00750

RRID

AB_3081600

文献引用格式

tPA/PLAT Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02965-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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