Anti-XAF1 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC FCM ELISA

A03432-1

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

加入购物车立即购买

Figure 1. Western blot analysis of anti- XAF1 antibody (A03432-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: 293T whole cell lysates,
Lane 3: Jurkat whole cell lysates.
Use rabbit anti- XAF1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for XAF1 at approximately 35KD. The expected band size for XAF1 is at 35KD.
all(5)
Figure 2. IHC analysis using anti- XAF1 antibody (A03432-1). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(5)
Figure 3. IHC analysis using anti- XAF1 antibody (A03432-1). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(5)
Figure 4. IHC analysis using anti- XAF1 antibody (A03432-1). detected in paraffin-embedded section of human Thyroid cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(5)
Figure 5. Flow cytometry analysis of U87 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(5)
all(5)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-XAF1 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

XAF1; BIRC4BP; XIAPAF1; BIRC4-binding protein

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, FCM, ELISA

基因名称

XAF1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human XAF1 recombinant protein (Position: Q197-S301).

计算分子量

35 kDa

实际分子量

34 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

XAF1 also called XIAP-associated factor 1,antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. By genomic sequence analysis, the XAF1 gene contains 7 exons spanning 18 kb. Southern blot analysis suggested that XAF1 is a single-copy gene. The XAF1 gene is mapped on 17p13.1. Fluorescence microscopy demonstrated nuclear expression of XAF1, in contrast to the cytoplasmic expression of XIAP. Functional analysis indicated that XAF1 reverses the XIAP-mediated inhibition of CASP3 and its protection against apoptosis. Northern blot analysis detected wide expression of 3.9-, 4.5-, 6.0-, and 7.0-kb XAF1 transcripts, with highest levels in heart and ovary and lowest levels in brain and testis. Antisense experiments determined that loss of XAF1 expression enhances resistance to apoptosis. Alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

Uniprot ID

Q6GPH4

文献引用格式

XAF1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A03432-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

XIAP associated factor 1

蛋白名全称

XIAP-associated factor 1

实验方案>

实验步骤

引用文献(0)>

清除