Figure 1. Western blot analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody (A03522). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: rat RH-35 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse Hepa1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 antigen affinity purified polyclonal antibody (A03522) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LAMC1/LAMC2/LAMC3 at approximately 150, 220-250 kDa. The expected band size for LAMC1/LAMC2/LAMC3 is at 177 kDa.
Figure 2. IHC analysis of Laminin using anti-Laminin antibody (A03522).Laminin was detected in paraffin-embedded section of mouse heart tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(8) 
Figure 3. IHC analysis of Laminin using anti-Laminin antibody (A03522).Laminin was detected in paraffin-embedded section of mouse kidney tissue .Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(8) 
Figure 4. IHC analysis of Laminin using anti-Laminin antibody (A03522).Laminin was detected in paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(8) 
Figure 5. IHC analysis of Laminin using anti-Laminin antibody (A03522).Laminin was detected in paraffin-embedded section of rat kidney tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(8) 
Figure 6. IF analysis using anti- Laminin antibody (A03522). detected in paraffin-embedded section of mouse skeletal muscle tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
all(8) 
Figure 7. IF analysis using anti- Laminin antibody (A03522). detected in paraffin-embedded section of mouse skeletal muscle tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
all(8) 
Figure 8. IF analysis using anti- Laminin antibody (A03522). detected in paraffin-embedded section of rat skeletal muscle tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
all(8) | Western blot (WB): | 1:1000-5000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody (A03522). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: rat RH-35 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse Hepa1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 antigen affinity purified polyclonal antibody (A03522) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LAMC1/LAMC2/LAMC3 at approximately 150, 220-250 kDa. The expected band size for LAMC1/LAMC2/LAMC3 is at 177 kDa.
Figure 2. IHC analysis of Laminin using anti-Laminin antibody (A03522).Laminin was detected in paraffin-embedded section of mouse heart tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Laminin using anti-Laminin antibody (A03522).Laminin was detected in paraffin-embedded section of mouse kidney tissue .Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of Laminin using anti-Laminin antibody (A03522).Laminin was detected in paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of Laminin using anti-Laminin antibody (A03522).Laminin was detected in paraffin-embedded section of rat kidney tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IF analysis using anti- Laminin antibody (A03522). detected in paraffin-embedded section of mouse skeletal muscle tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 7. IF analysis using anti- Laminin antibody (A03522). detected in paraffin-embedded section of mouse skeletal muscle tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 8. IF analysis using anti- Laminin antibody (A03522). detected in paraffin-embedded section of rat skeletal muscle tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 1. Western blot analysis of LAMC1/LAMC2/LAMC3 using anti-LAMC1/LAMC2/LAMC3 antibody (A03522). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: rat RH-35 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse Hepa1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LAMC1/LAMC2/LAMC3 antigen affinity purified polyclonal antibody (A03522) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LAMC1/LAMC2/LAMC3 at approximately 150, 220-250 kDa. The expected band size for LAMC1/LAMC2/LAMC3 is at 177 kDa.
Figure 2. IHC analysis of Laminin using anti-Laminin antibody (A03522).Laminin was detected in paraffin-embedded section of mouse heart tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Laminin using anti-Laminin antibody (A03522).Laminin was detected in paraffin-embedded section of mouse kidney tissue .Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of Laminin using anti-Laminin antibody (A03522).Laminin was detected in paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of Laminin using anti-Laminin antibody (A03522).Laminin was detected in paraffin-embedded section of rat kidney tissue . Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IF analysis using anti- Laminin antibody (A03522). detected in paraffin-embedded section of mouse skeletal muscle tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 7. IF analysis using anti- Laminin antibody (A03522). detected in paraffin-embedded section of mouse skeletal muscle tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 8. IF analysis using anti- Laminin antibody (A03522). detected in paraffin-embedded section of rat skeletal muscle tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
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