Figure 1. Western blot analysis of COX4I1 using anti-COX4I1 antibody (A05442-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COX4I1 antigen affinity purified polyclonal antibody (A05442-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COX4I1 at approximately 17 kDa. The expected band size for COX4I1 is at 20 kDa.
Figure 2. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of mouse small intestine tissue. rabbit anti-COX IV Antibody (A05442-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(11) 
Figure 3. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat liver tissue. rabbit anti-COX IV Antibody (A05442-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(11) 
Figure 4. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat lung tissue. rBiotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(11) 
Figure 5. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(11) 
Figure 6. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(11) 
Figure 7. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human placenta tissue. rBiotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(11) 
Figure 8. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human thyroid cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(11) 
Figure 9. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of mouse kidney tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(11) 
Figure 10. ICC analysis of anti-COX4I1 antibody (A05442-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and counterstained with DAPI (blue).
all(11) 
Figure 11. Flow cytometry analysis of U937 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(11) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of COX4I1 using anti-COX4I1 antibody (A05442-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COX4I1 antigen affinity purified polyclonal antibody (A05442-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COX4I1 at approximately 17 kDa. The expected band size for COX4I1 is at 20 kDa.
Figure 2. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of mouse small intestine tissue. rabbit anti-COX IV Antibody (A05442-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat liver tissue. rabbit anti-COX IV Antibody (A05442-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat lung tissue. rBiotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 7. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human placenta tissue. rBiotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 8. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human thyroid cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 9. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of mouse kidney tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 10. ICC analysis of anti-COX4I1 antibody (A05442-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and counterstained with DAPI (blue).
Figure 11. Flow cytometry analysis of U937 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of COX4I1 using anti-COX4I1 antibody (A05442-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COX4I1 antigen affinity purified polyclonal antibody (A05442-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COX4I1 at approximately 17 kDa. The expected band size for COX4I1 is at 20 kDa.
Figure 2. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of mouse small intestine tissue. rabbit anti-COX IV Antibody (A05442-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat liver tissue. rabbit anti-COX IV Antibody (A05442-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat lung tissue. rBiotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 7. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human placenta tissue. rBiotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 8. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of human thyroid cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 9. IHC analysis of COX IV using anti-COX IV antibody (A05442-1).COX IV was detected in paraffin-embedded section of mouse kidney tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 10. ICC analysis of anti-COX4I1 antibody (A05442-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and counterstained with DAPI (blue).
Figure 11. Flow cytometry analysis of U937 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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