Western blot (WB): | 1:500-2000 |
ELISA(Cap): | 1:50-1:200 |
Figure 1. Western blot analysis of OIF using anti-OIF antibody (A07061-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse OGN protein 1ng. anti-OIF antigen affinity purified polyclonal antibody (Catalog # A07061-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for OIF at approximately 34KD. The expected band size for OIF is at 34KD.
Figure 1. Western blot analysis of OIF using anti-OIF antibody (A07061-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse OGN protein 1ng. anti-OIF antigen affinity purified polyclonal antibody (Catalog # A07061-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for OIF at approximately 34KD. The expected band size for OIF is at 34KD.