Anti-DCLK3 Antibody

Rabbit polyclonal antibody

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A15882-1

0.1mg ￥5388
货期： 3-4周

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Western blot analysis of DCLK3 in K562 cell lysate with DCLK3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of DLCK3 in human brain tissue with DLCK3 antibody at 20 μg/mL.
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Immunohistochemistry of DCLK3 in mouse brain tissue with DCLK3 Antibody at 5 μg/mL.
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Immunofluorescence of DCLK3 in mouse brain tissue with DCLK3 Antibody at 20 μg/mL.

Green: DCLK3 antibody (A15882-1)
Red: Phylloidin staining
Blue: DAPI staining
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产品名称

Anti-DCLK3 Antibody

规格/价格

0.1mg/5388

指标别称

CLR; DCK3; DCDC3C; DCAMKL3; KIAA1765; Doublecortin domain-containing protein 3C

产品类型

Polyclonal

检验物种

human, mouse

应用范围

ELISA, WB, IHC, IF

基因名称

DCLK3

宿主

Rabbit

抗体亚型

IgG

免疫原

DCLK3 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human DCLK3. The immunogen is located within the last 50 amino acids of DCLK3.

计算分子量

74 kDa

成分

DCLK3 Antibody is supplied in PBS containing 0.02% sodium azide.

纯化方式

DCLK3 Antibody is affinity chromatography purified via peptide column.

浓度

1 mg/mL

产品形态

Liquid

保存条件

DCLK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

背景资料

DCLK3 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK3 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK3 transcripts are present in liver and kidney, suggesting that DCLK3 may play other roles than in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization.

Uniprot ID

Q9C098

文献引用格式

DCLK3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A15882-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Doublecortin-like kinase 3

蛋白名全称

Doublecortin-like kinase 3

实验方案>

实验步骤

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