产品名称
Anti-GFP tag Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
CFP; eGFP; eYFP; GFP; GFP tag; YFP
检验物种
aequoreavictoria, recombinantprotein
应用范围
WB, ICC/IF, IP, ELISA
免疫原
E.coli-derived human GFP tag recombinant protein (Position: G4-K239).
成分
500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
保存条件
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Green fluorescent protein(GFP) has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.
文献引用格式
GFP tag Antibody (Boster Biological Technology, Wuhan, China. Catalog#A30939)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
蛋白名全称
Green fluorescent protein
推荐稀释比
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| ImmunoPrecipitation (IP): | 1:50 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
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