Anti-Alpha E-Catenin/CTNNA1 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC

BA0425

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of Anti-CTNNA1 antibody (BA0425). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: MCF-7 whole cell lysates,Use rabbit Anti-CTNNA1 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CTNNA1 at approximately 100KD. The expected band size for CTNNA1 is at 100KD.
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Figure 2. IHC analysis using Anti-CTNNA1 antibody (BA0425) detected in paraffin-embedded section of mammary cancer tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Alpha E-Catenin/CTNNA1 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

alpha catenin; Alpha E catenin; Alpha E-Catenin; Cadherin associated protein; CAP102; Catenin alpha 1; CTNNA1; α-catenin; α-E-catenin; αE-catenin

产品类型

Polyclonal

检验物种

human, mouse, rabbit, rat

应用范围

WB,IHC

基因名称

CTNNA1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CTNNA1 recombinant protein (Position: D143-D292). Human CTNNA1 shares 98% amino acid (aa) sequence identity with mouse CTNNA1.

计算分子量

100 kDa

实际分子量

100 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CTNNA1, also known as Catenin alpha-1 or Catenin (cadherin-associated protein), alpha 1, is a protein that in humans is encoded by the CTNNA1 gene. It is mapped to 5q31.2. When surface epithelium CTNNA1 was ablated, hair follicle development was blocked and epidermal morphogenesis was dramatically affected, with defects in adherens junction formation, intercellular adhesion, and epithelial polarity. In vitro, CTNNA1 null keratinocytes were poorly contact inhibited and grew rapidly. These differences were not dependent upon intercellular adhesion and were in marked contrast to keratinocytes conditionally null for another essential intercellular adhesion protein, desmoplakin Knockout keratinocytes exhibited sustained activation of the Ras-MAPK cascade due to aberrations in growth factor responses. It is concluded that features of precancerous lesions often attributed to defects in cell cycle regulatory genes can be generated by compromising the function of CTNNA1.

Uniprot ID

P35221

文献引用格式

Alpha E-Catenin/CTNNA1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0425)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

Western blot (WB):	1:500-2000
Immunohistochemistry (IHC):	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Anti-Alpha E-Catenin/CTNNA1 Antibody

筛选器： All WB IHC

BA0425

产品简介>

产品名称

规格/价格

指标别称

产品类型

检验物种

应用范围

基因名称

抗体来源

抗体亚型

免疫原

计算分子量

实际分子量

成分

纯化方式

浓度

产品形态

保存条件

背景资料

Uniprot ID

文献引用格式

应用释义

推荐配套的二抗和检测试剂

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蛋白名全称

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Anti-Alpha E-Catenin/CTNNA1 Antibody

筛选器： All WB IHC

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