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Anti-Adrenomedullin/ADM Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA0629

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of Adrenomedullin/ADM using anti-Adrenomedullin/ADM antibody (BA0629). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: Rat brain tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Adrenomedullin/ADM antigen affinity purified polyclonal antibody (BA0629) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Adrenomedullin/ADM at approximately 20 kDa. The expected band size for Adrenomedullin/ADM is at 20 kDa.

    all(2)
  • IHC analysis of Adrenomedullin/ADM using anti-Adrenomedullin/ADM antibody (BA0629).
    Adrenomedullin/ADM was detected in a paraffin-embedded section of ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Adrenomedullin/ADM Antibody (BA0629) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(2)

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产品名称
Anti-Adrenomedullin/ADM Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
ADM; adrenomedullin; AM; PAMP; ProAM N terminal 20 peptide; ProAM N20
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
ADM
宿主
Rabbit
抗体亚型
IgG
免疫原
Polypeptide
计算分子量
20 kDa
实际分子量
20 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Adrenomedullin, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. Genomic ADM DNA consists of 4 exons and 3 introns, with the 5-prime flanking region containing TATA, CAAT, and GC boxes. There are also multiple binding sites for activator protein-2 and a cAMP-regulated enhancer element. [provided by RefSeq, Jul 2008]
Uniprot ID
P35318  
文献引用格式
Adrenomedullin/ADM Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0629)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Adrenomedullin/ADM Antibody

    筛选器: All WB IHC

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