产品名称
Biotin Conjugated AffiniPure Donkey Anti-mouse IgM μ Chain
纯化方式
The antibody was purified from antisera by immunoaffinity chromatography.
产品形态
Concentrated, Liquid
成分
1 mg/ml of biotin conjugated specific antibody; 0.01M PBS, 50% glycerol.
特异性
The antibody reacts with the heavy chain of mouse IgMbut not with mouse IgG or the light chains of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. With mouse IgG or the light chains of mouse immunoglobulins. shows no cross-reactivity with human, bovine, horse and rat serumproteins, but it may cross- react with IgM from other species .
应用注释
This antibody must be used with various kinds of enzymes or fluorescence conjugated avidin (streptavidin) in IHC and ELISA.Diluted by neutral PBS or TBS.
文献引用格式
Biotin Conjugated AffiniPure Donkey Anti-mouse IgM μ Chain (Boster Biological Technology, Wuhan, China. Catalog#BA1017)
建议工作浓度
Immunohistochemistry: 1:100-200;
ELISA: 1:5000—20000;
Optimal working dilutions must be determined by end users.
保存条件
At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.