Figure 1. Western blot analysis of anti- LMNB1 antibody (BA1228). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human THP-1 whole cell lvsates,
Lane 3: human HL-60 whole cell lvsates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates.
Use rabbit anti- LMNB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for LMNB1 at approximately 70KD. The expected band size for LMNB1 is at 70KD.
Figure 2. IHC analysis using anti- LMNB1 antibody (BA1228). detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 3.IHC analysis using anti- LMNB1 antibody (BA1228). detected in frozen section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 4. ICC analysis of LMNB1 using anti- LMNB1 antibody (BA1228).
LMNB1 was detected in an immunocytochemical section of A549 cells. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. ICC analysis using anti- LMNB1 antibody (BA1228) and anti-Tubulin beta antibody (M01857-3). were detected in immersion fixed SiHa cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).
all(6) 
Figure 6. ICC analysis using anti- LMNB1 antibody (BA1228). was detected in immersion fixed SiHa cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and counterstained with DAPI (blue).
all(6) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry in fixed cells (ICC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- LMNB1 antibody (BA1228). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human THP-1 whole cell lvsates,
Lane 3: human HL-60 whole cell lvsates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates.
Use rabbit anti- LMNB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for LMNB1 at approximately 70KD. The expected band size for LMNB1 is at 70KD.
Figure 2. IHC analysis using anti- LMNB1 antibody (BA1228). detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3.IHC analysis using anti- LMNB1 antibody (BA1228). detected in frozen section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. ICC analysis of LMNB1 using anti- LMNB1 antibody (BA1228).
LMNB1 was detected in an immunocytochemical section of A549 cells. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. ICC analysis using anti- LMNB1 antibody (BA1228) and anti-Tubulin beta antibody (M01857-3). were detected in immersion fixed SiHa cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 6. ICC analysis using anti- LMNB1 antibody (BA1228). was detected in immersion fixed SiHa cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and counterstained with DAPI (blue).
Figure 1. Western blot analysis of anti- LMNB1 antibody (BA1228). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human THP-1 whole cell lvsates,
Lane 3: human HL-60 whole cell lvsates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates.
Use rabbit anti- LMNB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for LMNB1 at approximately 70KD. The expected band size for LMNB1 is at 70KD.
Figure 2. IHC analysis using anti- LMNB1 antibody (BA1228). detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3.IHC analysis using anti- LMNB1 antibody (BA1228). detected in frozen section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. ICC analysis of LMNB1 using anti- LMNB1 antibody (BA1228).
LMNB1 was detected in an immunocytochemical section of A549 cells. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. ICC analysis using anti- LMNB1 antibody (BA1228) and anti-Tubulin beta antibody (M01857-3). were detected in immersion fixed SiHa cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).
Figure 6. ICC analysis using anti- LMNB1 antibody (BA1228). was detected in immersion fixed SiHa cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and counterstained with DAPI (blue).
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