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Anti-SYN1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA1421

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of SYN1 using anti-SYN1 antibody (BA1421). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat brain tissue lysates,
    Lane 2: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SYN1 antigen affinity purified polyclonal antibody (BA1421) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SYN1 at approximately 74 kDa. The expected band size for SYN1 is at 74 kDa.

    all(3)
  • IHC analysis of SYN1 using anti-SYN1 antibody (BA1421) .
    SYN1 was detected in a paraffin-embedded section of human cerebellum tissue. The tissue section was incubated with rabbit anti-SYN1 Antibody (BA1421) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(3)
  • IHC analysis of SYN1 using anti-SYN1 antibody (BA1421) .
    SYN1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SYN1 Antibody (BA1421) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(3)

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产品名称
Anti-SYN1 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Brain protein 4.1; SYN1; SYN1a; SYN1b; SYN1-Specific; Synapsin 1; synapsin I; SYNI
产品类型
Polyclonal
检验物种
human, mouse, rat, rabbit
应用范围
WB, IHC
基因名称
SYN1
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human Synapsin I identical to the related mouse and rat sequences.
计算分子量
74 kDa
实际分子量
74 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Synapsin I, is the collective name for Synapsin Ia and Synapsin Ib, two nearly identical phosphoproteins that in humans are encoded by the SYN1 gene. This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Uniprot ID
P17600  
文献引用格式
SYN1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1421)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
synapsin I
蛋白名全称
Synapsin-1
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-SYN1 Antibody

    筛选器: All WB IHC

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