Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of anti-TNC antibody (BA1428-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat brain tissue lysates,Lane 2: Rat lliver tissue lysates,Lane 3:Rat spleen tissue lysates,Use rabbit anti- TNC 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TNC at approximately 241KD. The expected band size for TNC is at 232KD.
Figure 1. Western blot analysis of anti-TNC antibody (BA1428-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat brain tissue lysates,Lane 2: Rat lliver tissue lysates,Lane 3:Rat spleen tissue lysates,Use rabbit anti- TNC 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TNC at approximately 241KD. The expected band size for TNC is at 232KD.