Figure 1. Western blot analysis of anti- HSPD1 antibody (BA1511).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1:Rat brain tissue lysates,Lane 2:Rat liver tissue lysates,Lane 3: Human CEM whole cell lysates,Lane 4: Human HELA whole cell lysates,Lane 5: Human SMMC whole cell lysates,Lane 6: Human COLO-320 whole cell lysates.Use rabbit anti- HSPD1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HSPD1 at approximately 60KD. The expected band size for HSPD1 is at 60KD.
all(6)
Figure 2. IHC analysis using anti- HSPD1 antibody (BA1511).detected in paraffin-embedded section of Rat Intestine Tissue tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 3. IHC analysis using anti- HSPD1 antibody (BA1511).detected in paraffin-embedded section of SMMC Cell tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 4. IHC analysis using anti- HSPD1 antibody (BA1511). detected in frozen section of Rat Intestine Tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 5. ICC analysis using anti- HSPD1 antibody (BA1511). was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
all(6) 
Figure 6. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(6) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- HSPD1 antibody (BA1511).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1:Rat brain tissue lysates,Lane 2:Rat liver tissue lysates,Lane 3: Human CEM whole cell lysates,Lane 4: Human HELA whole cell lysates,Lane 5: Human SMMC whole cell lysates,Lane 6: Human COLO-320 whole cell lysates.Use rabbit anti- HSPD1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HSPD1 at approximately 60KD. The expected band size for HSPD1 is at 60KD.
Figure 2. IHC analysis using anti- HSPD1 antibody (BA1511).detected in paraffin-embedded section of Rat Intestine Tissue tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis using anti- HSPD1 antibody (BA1511).detected in paraffin-embedded section of SMMC Cell tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis using anti- HSPD1 antibody (BA1511). detected in frozen section of Rat Intestine Tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. ICC analysis using anti- HSPD1 antibody (BA1511). was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 6. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- HSPD1 antibody (BA1511).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1:Rat brain tissue lysates,Lane 2:Rat liver tissue lysates,Lane 3: Human CEM whole cell lysates,Lane 4: Human HELA whole cell lysates,Lane 5: Human SMMC whole cell lysates,Lane 6: Human COLO-320 whole cell lysates.Use rabbit anti- HSPD1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HSPD1 at approximately 60KD. The expected band size for HSPD1 is at 60KD.
Figure 2. IHC analysis using anti- HSPD1 antibody (BA1511).detected in paraffin-embedded section of Rat Intestine Tissue tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis using anti- HSPD1 antibody (BA1511).detected in paraffin-embedded section of SMMC Cell tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis using anti- HSPD1 antibody (BA1511). detected in frozen section of Rat Intestine Tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. ICC analysis using anti- HSPD1 antibody (BA1511). was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 6. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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