Figure 1. Western blot analysis of Anti-OCT-2(POU2F2) antibody (BA1532). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human Hela whole cell lysates,Use rabbit Anti-OCT-2(POU2F2) 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for OCT-2(POU2F2) at approximately 63KD. The expected band size for OCT-2(POU2F2) is at 63KD.
all(2)
Figure 2.IHC analysis using Anti-OCT-2(POU2F2) antibody (BA1532) detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(2) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of Anti-OCT-2(POU2F2) antibody (BA1532). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human Hela whole cell lysates,Use rabbit Anti-OCT-2(POU2F2) 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for OCT-2(POU2F2) at approximately 63KD. The expected band size for OCT-2(POU2F2) is at 63KD.
Figure 2.IHC analysis using Anti-OCT-2(POU2F2) antibody (BA1532) detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. Western blot analysis of Anti-OCT-2(POU2F2) antibody (BA1532). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human Hela whole cell lysates,Use rabbit Anti-OCT-2(POU2F2) 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for OCT-2(POU2F2) at approximately 63KD. The expected band size for OCT-2(POU2F2) is at 63KD.
Figure 2.IHC analysis using Anti-OCT-2(POU2F2) antibody (BA1532) detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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