Anti-MAPK1/3 Antibody

筛选器： All WB ICC/IF FCM

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  Figure 1. Western blot analysis of MAPK1/3 using anti-MAPK1/3 antibody (BA1657). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human K562 whole cell lysates,
  Lane 3: human A431 whole cell lysates,
  Lane 4: human U2OS whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat C6 whole cell lysates,
  Lane 7: mouse brain lysates,
  Lane 8: mouse Neuro-2a whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAPK1/3 antigen affinity purified polyclonal antibody (BA1657) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MAPK1/3 at approximately 38, 42 kDa. The expected band size for MAPK1/3 is at 41 kDa/43 kDa.

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  Figure 2. IF analysis of MAPK1/3 using anti-MAPK1/3 antibody (BA1657) and anti-Beta Tubulin antibody (M01857-3).
  MAPK1/3 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-MAPK1/3 Antibody (BA1657) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.

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  Figure 3. Flow Cytometry analysis of Hela cells using anti-MAPK1/3 antibody (BA1657).
  Overlay histogram showing Hela cells stained with BA1657 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAPK1/3 Antibody (BA1657) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Figure 1. Western blot analysis of MAPK1/3 using anti-MAPK1/3 antibody (BA1657). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human K562 whole cell lysates,
  Lane 3: human A431 whole cell lysates,
  Lane 4: human U2OS whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat C6 whole cell lysates,
  Lane 7: mouse brain lysates,
  Lane 8: mouse Neuro-2a whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAPK1/3 antigen affinity purified polyclonal antibody (BA1657) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MAPK1/3 at approximately 38, 42 kDa. The expected band size for MAPK1/3 is at 41 kDa/43 kDa.

• 

  Figure 2. IF analysis of MAPK1/3 using anti-MAPK1/3 antibody (BA1657) and anti-Beta Tubulin antibody (M01857-3).
  MAPK1/3 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-MAPK1/3 Antibody (BA1657) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.

• 

  Figure 3. Flow Cytometry analysis of Hela cells using anti-MAPK1/3 antibody (BA1657).
  Overlay histogram showing Hela cells stained with BA1657 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAPK1/3 Antibody (BA1657) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.