Anti-PTH1R Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

BA3170

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of PTH1R using anti-PTH1R antibody (BA3170). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: SKOV whole cell lysates,
Lane 2: U2OS whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: SMMC whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PTH1R antigen affinity purified polyclonal antibody (BA3170) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PTH1R at approximately 66 kDa. The expected band size for PTH1R is at 66 kDa.
all(3)
IHC analysis of PTH1R using anti-PTH1R antibody (BA3170).
PTH1R was detected in a paraffin-embedded section of human thyroid cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PTH1R Antibody (BA3170) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
all(3)

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产品名称

Anti-PTH1R Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

parathyroid hormone 1 receptor; PFE; PTH/PTHr receptor; PTH/PTHrP type I receptor; PTH1 receptor; PTH1R; PTHR; PTHR1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

PTH1R

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Parathyroid Hormone Receptor 1, identical to the related mouse and rat sequences.

计算分子量

66 kDa

实际分子量

66 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Parathyroid hormone/parathyroid hormone-related peptide receptor, also known as PTH1R, PTHR, is a protein that in humans is encoded by the PTH1R gene. PTHR is a member of a family of G protein-coupled receptors. PTHR gene was mapped to the vicinity of the 3p21.3-p21.2 boundary by PCR analysis of human/rodent somatic cell hybrid panels using oligonucleotide primers designed to amplify a portion of the gene from genomic DNA. This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. PTH1R functions as a receptor for parathyroid hormone and for parathyroid hormone-related protein.

Uniprot ID

Q03431

文献引用格式

PTH1R Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3170)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

parathyroid hormone 1 receptor

蛋白名全称

Parathyroid hormone/parathyroid hormone-related peptide receptor

实验方案>

实验步骤

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