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Anti-SLC30A4 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA3484

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 3-4周
  • Western blot analysis of SLC30A4 using anti-SLC30A4 antibody (BA3484). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: MCF-7 whole cell lysates,
    Lane 2: A549 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SLC30A4 antigen affinity purified polyclonal antibody (BA3484) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SLC30A4 at approximately 47 kDa. The expected band size for SLC30A4 is at 47 kDa.

    all(3)
  • IHC analysis of SLC30A4 using anti-SLC30A4 antibody (BA3484).
    SLC30A4 was detected in a paraffin-embedded section of rat pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SLC30A4 Antibody (BA3484) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(3)
  • IHC analysis of SLC30A4 using anti-SLC30A4 antibody (BA3484).
    SLC30A4 was detected in a paraffin-embedded section of rat gaster tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SLC30A4 Antibody (BA3484) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(3)

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产品名称
Anti-SLC30A4 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
SLC30A4; Zinc transporter 4; ZnT 4; ZNT4; ZnT-4
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
SLC30A4
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human SLC30A4(407-427aa TIQLQSYRQEVDRTCANCQSS), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
计算分子量
47 kDa
实际分子量
47 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Zinc transporter 4, also called SLC30A4 or ZNT4 is a protein that in humans is encoded by the SLC30A4 gene. SLC30A4, or ZNT4 belongs to the ZNT family of zinc transporters. This gene is mapped to 15q21.1. Zinc is the second most abundant trace metal in the human body. It is an essential element, serving both a structural role, as in the formation of zinc fingers in DNA-binding proteins, and a catalytic role in metalloenzymes, such as pancreatic carboxypeptidases, alkaline phosphatases, various dehydrogenases, and superoxide dismutases. ZNTs are involved in transporting zinc out of the cytoplasm and have similar structures, consisting of 6 transmembrane domains and a histidine-rich cytoplasmic loop.
Uniprot ID
O14863  
文献引用格式
SLC30A4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3484)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
solute carrier family 30(zinc transporter), member 4
蛋白名全称
Zinc transporter 4
推荐稀释比
Western blot(WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-SLC30A4 Antibody

    筛选器: All WB IHC

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