Anti-NOX1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA3720

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-NOX1 antibody (BA3720). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain whole cell lysates,
Lane 2: mouse brain whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOX1 antigen affinity purified polyclonal antibody (BA3720) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NOX1 at approximately 65 kDa. The expected band size for NOX1 is at 65 kDa.
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Figure 2. IHC analysis of NOX1 using anti-NOX1 antibody (BA3720).
NOX1 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-NOX1 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

GP91 2; Mitogenic oxidase 1; MOX 1; MOX1; NADPH oxidase 1; NOH 1; NOH1; NOX 1; NOX1

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB,IHC

基因名称

NOX1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of rat NOX1, different from the related mouse sequence by one amino acid.

计算分子量

65 kDa

实际分子量

65 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

NOX1(NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.

Uniprot ID

Q9WV87

文献引用格式

NOX1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3720)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

NADPH oxidase 1

蛋白名全称

NADPH oxidase 1(NOX-1)

实验方案>

实验步骤

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