Western blot (WB): | 1:500-2000 |
Western blot analysis of TNF Beta/LTA using anti-TNF Beta/LTA antibody (BA4905).
Lane 1: recombinant human TNFβ protein 10ng,
Lane 2: recombinant human TNFβ protein 5ng,
Lane 3: recombinant human TNFβ protein 2.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TNF Beta/LTA antigen affinity purified polyclonal antibody (BA4905) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TNF Beta/LTA at approximately 22 kDa.
Western blot analysis of TNF Beta/LTA using anti-TNF Beta/LTA antibody (BA4905).
Lane 1: recombinant human TNFβ protein 10ng,
Lane 2: recombinant human TNFβ protein 5ng,
Lane 3: recombinant human TNFβ protein 2.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TNF Beta/LTA antigen affinity purified polyclonal antibody (BA4905) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TNF Beta/LTA at approximately 22 kDa.