Anti-AKT1 Antibody (Clone#PKB-175)

Mouse monoclonal antibody

说明书

筛选器： All WB

BM1612

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- AKT1 antibody (BM1612). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse brain tissue lysates.
Use mouse anti- AKT1 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1001). A specific band was detected for AKT1 at approximately 60KD. The expected band size for AKT1 is at 60KD.
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产品简介>

产品名称

Anti-AKT1 Antibody (Clone#PKB-175)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

AKT; AKT1; PKB; PKB ALPHA; PRKBA; Protein kinase B; Proto oncogene c Akt; RAC; RAC ALPHA; RAC PK alpha

产品类型

Monoclonal

检验物种

human, mouse, rat, chicken

应用范围

基因名称

AKT1

克隆号

PKB-175

抗体来源

Mouse

抗体亚型

IgG1

免疫原

Polypeptide

计算分子量

60 kDa

实际分子量

60 kDa

成分

200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA

纯化方式

Ascites

浓度

200ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.

背景资料

PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1(ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.

Uniprot ID

Human(P31749), Mouse(P31750), Rat(P47196)

文献引用格式

AKT1 Antibody (Clone#PKB-175) (Boster Biological Technology, Wuhan, China. Catalog#BM1612)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

v-akt murine thymoma viral oncogene homolog 1

蛋白名全称

RAC-alpha serine/threonine-protein kinase

实验方案>

实验步骤

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