HRP Conjugated IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific

筛选器： All WB Dot blot ELISA

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  IP analysis of DBT using anti-DBT antibody (A01145-2) in A431 whole cell lysate.
  Western blot analysis of DBT using anti- DBT antibody (A01145-2).
  Lane 1: A431 whole cell lysates(30ug),
  Lane 2: Rabbit control IgG instead of anti- DBT antibody in A431 whole cell lysate,
  Lane 3: anti- DBT antibody (2μg) + A431 whole cell lysate (500μg).
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- DBT antigen affinity purified polyclonal antibody (A01145-2) at a dilution of 1:1000 and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DBT at approximately 53 kDa. The expected band size for DBT is at 53 kDa.

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  IP analysis of DBT using anti-DBT antibody (A01145-2) in A431 whole cell lysate.
  Western blot analysis of DBT using anti- DBT antibody (A01145-2).
  Lane 1: A431 whole cell lysates(30ug),
  Lane 2: Rabbit control IgG instead of anti- DBT antibody in A431 whole cell lysate,
  Lane 3: anti- DBT antibody (2μg) + A431 whole cell lysate (500μg).
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- DBT antigen affinity purified polyclonal antibody (A01145-2) at a dilution of 1:1000 and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DBT at approximately 53 kDa. The expected band size for DBT is at 53 kDa.

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